Search results for "spettroemtria di massa"

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Characterization of heat-labile toxin-subunit B from Escherichia coli by liquid chromatography-electrospray ionization-mass spectrometry and matrix-a…

2012

The possibilities of characterizing the heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli (ETEC) by liquid chromatography electrospray mass spectrometry (LC/ESI-MS) and matrix-assisted laser desorption with time-of-flight mass spectrometry (MALDI-TOF-MS) were investigated. The B subunit from recombinant E. coli (expression in Pichia pastoris) can be detected by LC/ESI-MS expressed in P. pastoris and the charge envelope signals can be observed; LC/ESI-MS and MALDI-TOF-MS analysis allowed the acquisition of labile toxin subunit B (LTB) molecular weight and preliminary structural characterization of LTB toxin. MALDI-TOF analysis after reduction and alkylation of the protein evid…

Spectrometry Mass Electrospray IonizationElectrospray ionizationProtein subunitBacterial ToxinsMolecular Sequence DataToxicologyMass spectrometrymedicine.disease_causespettroemtria di massaPichiaPichia pastorisEnterotoxinsProtein sequencingEnterotoxigenic Escherichia colimedicineTrypsinAmino Acid SequenceDisulfidesPhosphorylationEscherichia colitossinaChromatographyMolecular massbiologyChemistryEscherichia coli ProteinsE. coliGeneral Medicinebiology.organism_classificationRecombinant ProteinsMolecular WeightProtein SubunitsSpectrometry Mass Matrix-Assisted Laser Desorption-IonizationFood ScienceChromatography LiquidFood and chemical toxicology : an international journal published for the British Industrial Biological Research Association
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